Composite

Part:BBa_M1115:Design

Designed by: Joshua Ellis   Group: UtahState_BE5930_-_S11   (2011-04-17)


Final lignin degradation construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4659
    Illegal XhoI site found at 1416
    Illegal XhoI site found at 2943
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1530
    Illegal NgoMIV site found at 2815
    Illegal NgoMIV site found at 2848
    Illegal NgoMIV site found at 2890
    Illegal NgoMIV site found at 4059
    Illegal NgoMIV site found at 4575
    Illegal NgoMIV site found at 4627
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1136
    Illegal BsaI site found at 4117
    Illegal BsaI.rc site found at 3485


Design Notes

None


Source

Promoter, RBS, and terminator parts came from the registry. The coding sequences came from genomic sequences found within the NCBI and JGI databases.

References